Protein Method Blocking Buffers
Biotium 10X Fish Gelatin Blocking Agent
10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.
LAB BIOREAGENTS StrongBlock III | 500mL
Lab Bioreagents Buffers have been developed through the screening of >2000 compounds with the support of machine learning (AI) and optimized through kinetic and dynamic binding tests. StrongBlock (I, II, III) are newly developed blocking buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The blocking buffer is to block excess binding sites on the micro-plate for reducing non-specific binding signal, by which the assay sensitivity and specificity are improved. As many factors can influence nonspecific binding, there is no single blocking buffer that is ideal for each assay. For example, depending on the recognition of detection probes from different assays, one individual blocking buffer may cross-react with detection probe. StrongBlock (I, II, III) are a variety of buffers designed by different blocker components.
Santa Cruz Biotechnology UltraCruz Blocking Reagent, 100 mL
Plant protein derived universal antibody diluent and blocking reagent recommended for use in immunohistochemistry (IHC), immunofluorescence (IF), Western blotting (WB), and for other research applications. Animal-free alternative to sera, BSA or non-fat dry milk. Designed to stabilize diluted antibody solutions and minimize background due to non-specific binding in different antibody-based research applications. The UltraCruz Blocking Reagent is especially convenient in multiplex IHC (multiple antigen labeling) applications where antibodies from several different species are used.
LI COR BIOTECH LLC TBS Blocking Buffer and PVDF Kits (single buffer kit)
The non-mammalian Intercept Blocker in these kits is in TBS (Tris-buffered saline) containing 0.06% Kathon II. The Immobilon-FL PVDF membrane included in these kits has been tested to ensure a low background for infrared Western blot success. This kit contains Intercept (TBS) Blocking Buffer (500 mL) and Millipore Immobilon-FL PVDF Membrane (1 roll; 0.45µm, 26.5 cm x 3.75 m).
Abcam ELISA Coating Buffer 1X, 120ML
ELISA Coating Buffer 1X (ab210899) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences Phosphate Buffered Saline with 0.05% TWEEN 20, pH 7.4, 10 Pack
Phosphate Buffered Saline with 0.05% TWEEN 20, pH 7.4, 10 Pack
LI COR BIOTECH LLC Intercept T20 (PBS) Antibody Diluent (3x500mL)
Intercept T20 (PBS) Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects.The diluent contains Intercept (PBS) Blocking Buffer preformulated with Tween 20 for use in a phosphate-buffered saline (PBS) system. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (PBS) Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
LI COR BIOTECH LLC Intercept T20 (PBS) Protein-Free Antibody Diluent (3x500mL)
Intercept T20 (PBS) Protein-Free Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (PBS) Protein-Free Blocking Buffer preformulated with Tween 20 for use in a phosphate-buffered saline (PBS) system. This diluent can be used where animal-based products are prohibited. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (PBS) Protein-Free Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Boston Bioproducts Inc Tris Buffered Saline-Tween, TBST (10X, with 0.5% Tween-20, pH 7.6) - 1L
10X Tris Buffered Saline - Tween (TBST) is a buffer-surfactant solution supporting a stable pH range between 7.4 and 8.0. When combined in a variable ratio, TBST is an effective wash buffer in Western blotting, in situ hybridization procedures, and immunohistochemistry staining, as it minimizes non specific binding of the materials without disrupting antigen-antibody binding. TBST is also used as an antibody diluent in immunoassays, and supports an array of molecular biology techniques.